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Pcr principles and procedure pdf WordPress.com

Real-time polymerase chain reaction Wikipedia

real time pcr principle pdf

Real time PCR using taqman dye YouTube. Real-Time PCR Handbook. An excellent resource for anyone who is new to real-time PCR and interested in learning the basic principles of the technology. This 3rd edition contains 6 chapters in 70 pages and also covers topics such as assay design, data analysis, real-time PCR tips, and troubleshooting., Applications of Real-Time PCR/qPCR Assays. Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis, the detection of genetically modified organisms in food, and cancer phenotyping..

Real-Time PCR Vs. Traditional PCR

Introduction to TaqManВ® and SYBRВ® Green Chemistries for. Thermo Fisher Scientific provides a full range of real-time PCR (qPCR) products and services to assist you in every step including qPCR assays, master mixes, reagents and instruments., PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications. In Sections 5–7, we present sample protocols and data that demonstrate the use of real-time PCR in ….

PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications. In Sections 5–7, we present sample protocols and data that demonstrate the use of real-time PCR in … Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications. This

UNIT 10.3 Real-Time PCR Dean Fraga,1 Tea Meulia,2 and Steven Fenster3 1 College of Wooster, Wooster, Ohio 2 Ohio Agricultural Research and Development Center, Wooster, Ohio 3 Ashland University, Ashland, Ohio 10 OVERVIEW AND PRINCIPLES The real-time polymerase chain reaction (real-time PCR) is a recent modification to PCR (UNIT 10.2) that is rapidly changing the nature of how biomedical Real Time PCR - Basic simple animation - part 1 intro HD.mp4 Specialized thermal cyclers equipped with fluorescence detection modules are used. The measured fluorescence is proportional to the total amount of amplicon; the change in fluorescence over time is used to calculate the amount of amplicon. How Does Real-Time PCR Work?

Real Time PCR - Basic simple animation - part 1 intro HD.mp4 Specialized thermal cyclers equipped with fluorescence detection modules are used. The measured fluorescence is proportional to the total amount of amplicon; the change in fluorescence over time is used to calculate the amount of amplicon. How Does Real-Time PCR Work? Real-Time PCR allows for direct measurement of the amount of the virus RNA in the patient. Virus RNA Real-Time Example: Determining percentage of GMO food PCR in Food content. Testing Determination of percent GMO food content important for import / export regulations. Labs use Real-Time PCR to measure amount of transgenic versus wild-type DNA.

Theory and explanation of Real-Time Polymerase Chain Reaction (adapted from Bustin et al 2012 An Introduction to the real-time polymerase chain reaction qPCR).. Traditionally, PCR is performed in a tube and when the reaction is complete the products of the reaction (the amplified DNA fragments) are analysed and visualised by gel electrophoresis. 9/11/2004 · Abstract. This review examines how real-time PCR can be used to determine copy number and zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies of a transgene or are hemizygous and homozygous requires the ability to routinely distinguish twofold differences, a detection difference which approaches the resolution of PCR-based quantification …

3/7/2016 · Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring … Real-Time PCR This same principle of amplification is employed in real-time PCR. But instead of looking at bands on a gel at the end of the reaction, the process is monitored in “real-time”. Literally, the reaction is placed in to a real-time PCR machine that watches the reaction occur with a camera or detector.

A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications. This

pdf. Real-time PCR. 362 Pages. Real-time PCR. Haitham Amer. Download with Google Download with Facebook or download with email. Real-time PCR. Download. Real-time PCR. 9/11/2004 · Abstract. This review examines how real-time PCR can be used to determine copy number and zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies of a transgene or are hemizygous and homozygous requires the ability to routinely distinguish twofold differences, a detection difference which approaches the resolution of PCR-based quantification …

Introduction to TaqMan ® and SYBR Green Chemistries for Real-Time PCR Reverse transcription-polymerase chain reaction (RT-PCR) Introduction to TaqMan 3 ® and SYBR® Green Chemistries for Real-Time PCR Protocol TaqMan ® Chemistry Reverse transcription-polymerase chain reaction (RT-PCR) RT-PCR is a method to amplify, or increase, the amount of PCR is a simple, versatile, sensitive, specific and reproducible assay. The following description of the principle of PCR is from Rodríguez-Lázaro, D. and Hernández, M. (2013). Introduction to the Real-time PCR published in Real-Time PCR in Food Science: Current Technology and Applications. D.

lifetechnologies.com 2 Basics of real-time PCR 1 1.1 Introduction 3 1.2 Overview of real-time PCR 4 1.3 Overview of real-time PCR and real-time PCR components 5 1.4 Real-time PCR analysis terminology 7 1.5 Real-time PCR fluorescence detection systems 11 1.6 Melting curve analysis 15 1.7 Use of passive reference dyes 16 1.8 Contamination prevention 17 real time pcr principle and procedure pdf file of this picture.and RT-PCR in an attempt to clarify the principles and. Other procedure after the DNA amplification. Procedure to amplify a target DNA segment in genomic DNA. Manualsppspcrtechguide.pdf. 1 PCR principles and procedure. 1

10/24/2013 · Real time PCR using taqman dye also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain … PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications. In Sections 5–7, we present sample protocols and data that demonstrate the use of real-time PCR in …

Reverse transcription polymerase chain reaction Wikipedia. Thermo Fisher Scientific provides a full range of real-time PCR (qPCR) products and services to assist you in every step including qPCR assays, master mixes, reagents and instruments., Overview of Real-Time PCR Principles 407 both aromatic systems, which conv ert electronic excitation energy into heat that dissipates to the surrounding solvent. On the other hand the dyes become brightly fluorescent when they bind to DNA, presumably to the minor groove, and ….

Roche Applied Science Technical Note

real time pcr principle pdf

(PDF) Basic principles of real-time quantitative PCR. Reaction is to make a huge number of copies of a. pdf file of this picture.The invention of Polymerase Chain Reaction PCR by K. In understanding the principles of PCR and its applications, the nature.To understand real time PCR it is easier to begin with the principles of a basic PCR: PCR is a technique for amplifying DNA. arms pcr principle pdf, PDF Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. basic principle of real-time PCR and is an. Basic principles of real-time.

Multiplex real time PCR pro’s and con’s. Introduction to TaqMan ® and SYBR Green Chemistries for Real-Time PCR Reverse transcription-polymerase chain reaction (RT-PCR) Introduction to TaqMan 3 ® and SYBR® Green Chemistries for Real-Time PCR Protocol TaqMan ® Chemistry Reverse transcription-polymerase chain reaction (RT-PCR) RT-PCR is a method to amplify, or increase, the amount of, Sarah Maddocks, Rowena Jenkins, in Understanding PCR, 2017. 4.1 Introduction. Quantitative polymerase chain reaction (Q-PCR) is a method by which the amount of the PCR product can be determined, in real-time, and is very useful for investigating gene expression..

Real-Time PCR Vs. Traditional PCR

real time pcr principle pdf

(PDF) Basic principles of real-time quantitative PCR. In contrast to regular reverse transcriptase-PCR and analysis by agarose gels, real-time PCR gives quantitative results. An additional advantage of real-time PCR is the relative ease and convenience of use compared to some older methods (as long as one has access to a suitable real-time PCR machine). https://fr.m.wikipedia.org/wiki/High_Resolution_Melt In contrast to regular reverse transcriptase-PCR and analysis by agarose gels, real-time PCR gives quantitative results. An additional advantage of real-time PCR is the relative ease and convenience of use compared to some older methods (as long as one has access to a suitable real-time PCR machine)..

real time pcr principle pdf


Sarah Maddocks, Rowena Jenkins, in Understanding PCR, 2017. 4.1 Introduction. Quantitative polymerase chain reaction (Q-PCR) is a method by which the amount of the PCR product can be determined, in real-time, and is very useful for investigating gene expression. PDF Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. basic principle of real-time PCR and is an. Basic principles of real-time

Progress of DNA amplification during a Polymerase Chain Reaction (PCR) can be monitored in "real time" (RT-PCR) by measuring the release of fluorescent "flashes" during amplification. Reaction rates can be measured continuously, or determined at a fixed time-point during the exponential amplification phase. 10/30/2009 · Real Time PCR overcome this problem, because of its ability to measure the PCR amplicons at early states of the reaction as they are accumulate in a “Real Time Detection” mode thus measuring the amount of PCR product where the reaction is still in the exponential phase (QPCR).

real time pcr principle and procedure pdf file of this picture.and RT-PCR in an attempt to clarify the principles and. Other procedure after the DNA amplification. Procedure to amplify a target DNA segment in genomic DNA. Manualsppspcrtechguide.pdf. 1 PCR principles and procedure. 1 10/30/2009 · Real Time PCR overcome this problem, because of its ability to measure the PCR amplicons at early states of the reaction as they are accumulate in a “Real Time Detection” mode thus measuring the amount of PCR product where the reaction is still in the exponential phase (QPCR).

pdf. Real-time PCR. 362 Pages. Real-time PCR. Haitham Amer. Download with Google Download with Facebook or download with email. Real-time PCR. Download. Real-time PCR. Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications. This

5/30/2018 · The advent of real-time PCR and real-time reverse transcription PCR (real-time RT-PCR) has dramatically changed the field of measuring gene expression. Real-time PCR is the technique of collecting data throughout the PCR process as it occurs, thus … 9/6/2018 · Real Time PCR- Principle, Process, Markers, Advantages, Applications. qPCR is a technique used to monitor the progress of a PCR reaction in real time.

10/24/2013 · Real time PCR using taqman dye also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain … analysis of real-time PCR data. Keywords Quantification.Expressionratio. PCRefficiency.TaqMan.SYBRGreen Introduction The quantification of gene-specific mRNA expression is one of the major issues in life science and basic clinical research. Since its establishment in the 1990s [1–4], quantitative real-time RT-PCR (qPCR) has been widely

Applications of Real-Time PCR/qPCR Assays. Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis, the detection of genetically modified organisms in food, and cancer phenotyping. Real-time PCR (SYBR green) protocol (Briana 6/2011) Principle: PCR technology is widely used for quantifying DNA because the amplification of the target sequence allows for greater sensitivity of detection than other methods. In an optimized reaction, the target quantity will approximately double during each amplification cycle.

•Multiplex real time PCR has many advantages. •Important to be aware of pitfalls associated with these assays •The pitfalls described here: –Can affect the performance of a test both within a lab and between laboratories. •Can affect the retrospective incorporation of IC component. real time pcr principle and procedure pdf file of this picture.and RT-PCR in an attempt to clarify the principles and. Other procedure after the DNA amplification. Procedure to amplify a target DNA segment in genomic DNA. Manualsppspcrtechguide.pdf. 1 PCR principles and procedure. 1

Sarah Maddocks, Rowena Jenkins, in Understanding PCR, 2017. 4.1 Introduction. Quantitative polymerase chain reaction (Q-PCR) is a method by which the amount of the PCR product can be determined, in real-time, and is very useful for investigating gene expression. 10/30/2009 · Real Time PCR overcome this problem, because of its ability to measure the PCR amplicons at early states of the reaction as they are accumulate in a “Real Time Detection” mode thus measuring the amount of PCR product where the reaction is still in the exponential phase (QPCR).

Real Time PCR Primer and Probe Design with AlleleID® & Beacon Designer™ Beacon Designer™ is a comprehensive real time PCR primer and probe design tool for designing single template and multiplex assays. Real time PCR chemistries supported include Molecular beacons, TaqMan®, FRET, Scorpions® and SYBR® Green. Real-time PCR (SYBR green) protocol (Briana 6/2011) Principle: PCR technology is widely used for quantifying DNA because the amplification of the target sequence allows for greater sensitivity of detection than other methods. In an optimized reaction, the target quantity will approximately double during each amplification cycle.

Principle of PCR. unit 10.3 real-time pcr dean fraga,1 tea meulia,2 and steven fenster3 1 college of wooster, wooster, ohio 2 ohio agricultural research and development center, wooster, ohio 3 ashland university, ashland, ohio 10 overview and principles the real-time polymerase chain reaction (real-time pcr) is a recent modification to pcr (unit 10.2) that is rapidly changing the nature of how biomedical, pdf real-time quantitative pcr allows the sensitive, specific and reproducible quantitation of nucleic acids. basic principle of real-time pcr and is an. basic principles of real-time).

•Multiplex real time PCR has many advantages. •Important to be aware of pitfalls associated with these assays •The pitfalls described here: –Can affect the performance of a test both within a lab and between laboratories. •Can affect the retrospective incorporation of IC component. Real-Time PCR Handbook. An excellent resource for anyone who is new to real-time PCR and interested in learning the basic principles of the technology. This 3rd edition contains 6 chapters in 70 pages and also covers topics such as assay design, data analysis, real-time PCR tips, and troubleshooting.

Overview of Real-Time PCR Principles 407 both aromatic systems, which conv ert electronic excitation energy into heat that dissipates to the surrounding solvent. On the other hand the dyes become brightly fluorescent when they bind to DNA, presumably to the minor groove, and … 10/24/2013 · Real time PCR using taqman dye also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain …

9/11/2004 · Abstract. This review examines how real-time PCR can be used to determine copy number and zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies of a transgene or are hemizygous and homozygous requires the ability to routinely distinguish twofold differences, a detection difference which approaches the resolution of PCR-based quantification … Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives This tutorial will provide an understanding of the following: Limitations of traditional PCR Introduction to Real-Time PCR

Thermo Fisher Scientific provides a full range of real-time PCR (qPCR) products and services to assist you in every step including qPCR assays, master mixes, reagents and instruments. 9/6/2018 · Real Time PCR- Principle, Process, Markers, Advantages, Applications. qPCR is a technique used to monitor the progress of a PCR reaction in real time.

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA. Real-Time PCR This same principle of amplification is employed in real-time PCR. But instead of looking at bands on a gel at the end of the reaction, the process is monitored in “real-time”. Literally, the reaction is placed in to a real-time PCR machine that watches the reaction occur with a camera or detector.

RT-PCR (Reverse Transcription PCR, Real Time - PCR) Used to reverse-transcribe and amplify RNA to cDNA. PCR is preceded by a reaction using reverse transcriptase, an enzyme that converts RNA into cDNA. The two reactions may be combined in a tube. Uses: 1-Detection of RNA virus like (HCV). Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives This tutorial will provide an understanding of the following: Limitations of traditional PCR Introduction to Real-Time PCR

PCR is a simple, versatile, sensitive, specific and reproducible assay. The following description of the principle of PCR is from Rodríguez-Lázaro, D. and Hernández, M. (2013). Introduction to the Real-time PCR published in Real-Time PCR in Food Science: Current Technology and Applications. D. 9/6/2018 · Real Time PCR- Principle, Process, Markers, Advantages, Applications. qPCR is a technique used to monitor the progress of a PCR reaction in real time.

real time pcr principle pdf

What is Real-Time PCR (qPCR)? LSR Bio-Rad

Pcr principles and procedure pdf WordPress.com. real-time quantitative pcr allows the sensitive, specific and reproducible quantitation of nucleic acids. since its introduction, real-time quantitative pcr has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications. this, progress of dna amplification during a polymerase chain reaction (pcr) can be monitored in "real time" (rt-pcr) by measuring the release of fluorescent "flashes" during amplification. reaction rates can be measured continuously, or determined at a fixed time-point during the exponential amplification phase.).

real time pcr principle pdf

Real-Time PCR Basics Real Time Polymerase Chain Reaction

www.primerdesign.co.uk. progress of dna amplification during a polymerase chain reaction (pcr) can be monitored in "real time" (rt-pcr) by measuring the release of fluorescent "flashes" during amplification. reaction rates can be measured continuously, or determined at a fixed time-point during the exponential amplification phase., real-time pcr handbook. an excellent resource for anyone who is new to real-time pcr and interested in learning the basic principles of the technology. this 3rd edition contains 6 chapters in 70 pages and also covers topics such as assay design, data analysis, real-time pcr tips, and troubleshooting.).

real time pcr principle pdf

Reverse transcription polymerase chain reaction Wikipedia

Real-Time Polymerase Chain Reaction an overview. real time pcr principle and procedure pdf file of this picture.and rt-pcr in an attempt to clarify the principles and. other procedure after the dna amplification. procedure to amplify a target dna segment in genomic dna. manualsppspcrtechguide.pdf. 1 pcr principles and procedure. 1, real-time throughput computer desktop laptop or desktop applied biosystems 7500 fast real-time pcr system-standard with rq -standard with rq-paid options: -enterprise-rq manager-snp manager software quantitation allelic discrimination plus/minus detection applications applied biosystems 7300 real-time pcr system applied biosystems 7500 real).

real time pcr principle pdf

Overview of Real-Time PCR Principles

Real Time PCR- Principle Process Markers Advantages. unit 10.3 real-time pcr dean fraga,1 tea meulia,2 and steven fenster3 1 college of wooster, wooster, ohio 2 ohio agricultural research and development center, wooster, ohio 3 ashland university, ashland, ohio 10 overview and principles the real-time polymerase chain reaction (real-time pcr) is a recent modification to pcr (unit 10.2) that is rapidly changing the nature of how biomedical, 5/30/2018 · the advent of real-time pcr and real-time reverse transcription pcr (real-time rt-pcr) has dramatically changed the field of measuring gene expression. real-time pcr is the technique of collecting data throughout the pcr process as it occurs, thus …).

real time pcr principle pdf

Basic principles of real-time quantitative PCR.

Roche Applied Science Technical Note. 11/18/2009 · realtime-pcr
21. what is real time pcr?
real time pcr is a technique in which fluoroprobes bind to specific target regions of amplicons to produce fluorescence during pcr.
the fluorescence, measured in real time, is detected in …, real time pcr primer and probe design with alleleid® & beacon designer™ beacon designer™ is a comprehensive real time pcr primer and probe design tool for designing single template and multiplex assays. real time pcr chemistries supported include molecular beacons, taqman®, fret, scorpions® and sybr® green.).

Introduction to TaqMan ® and SYBR Green Chemistries for Real-Time PCR Reverse transcription-polymerase chain reaction (RT-PCR) Introduction to TaqMan 3 ® and SYBR® Green Chemistries for Real-Time PCR Protocol TaqMan ® Chemistry Reverse transcription-polymerase chain reaction (RT-PCR) RT-PCR is a method to amplify, or increase, the amount of Real-Time PCR This same principle of amplification is employed in real-time PCR. But instead of looking at bands on a gel at the end of the reaction, the process is monitored in “real-time”. Literally, the reaction is placed in to a real-time PCR machine that watches the reaction occur with a camera or detector.

Real-Time PCR Handbook. An excellent resource for anyone who is new to real-time PCR and interested in learning the basic principles of the technology. This 3rd edition contains 6 chapters in 70 pages and also covers topics such as assay design, data analysis, real-time PCR tips, and troubleshooting. 5/1/2014 · Role of Real Time PCR Beside normal amplification process performed by normal PCR, Real Time PCR can perform detection, analysis and quantification of the sample. Detection: Find out the presence of targeted gene sequence which is assured by the presence of the amplification curve.

analysis of real-time PCR data. Keywords Quantification.Expressionratio. PCRefficiency.TaqMan.SYBRGreen Introduction The quantification of gene-specific mRNA expression is one of the major issues in life science and basic clinical research. Since its establishment in the 1990s [1–4], quantitative real-time RT-PCR (qPCR) has been widely basic principle of real-time PCR and is an essential component in producing accu-rate and reproducible data [1]. The pres-ence of more template at the start of the reaction leads to a fewer number of cycles reaching the point at which the fluores-cent signal is recorded as statistically sig-nificant above background [24]. This Ct

Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives This tutorial will provide an understanding of the following: Limitations of traditional PCR Introduction to Real-Time PCR pdf. Real-time PCR. 362 Pages. Real-time PCR. Haitham Amer. Download with Google Download with Facebook or download with email. Real-time PCR. Download. Real-time PCR.

Reaction is to make a huge number of copies of a. pdf file of this picture.The invention of Polymerase Chain Reaction PCR by K. In understanding the principles of PCR and its applications, the nature.To understand real time PCR it is easier to begin with the principles of a basic PCR: PCR is a technique for amplifying DNA. arms pcr principle pdf lifetechnologies.com 2 Basics of real-time PCR 1 1.1 Introduction 3 1.2 Overview of real-time PCR 4 1.3 Overview of real-time PCR and real-time PCR components 5 1.4 Real-time PCR analysis terminology 7 1.5 Real-time PCR fluorescence detection systems 11 1.6 Melting curve analysis 15 1.7 Use of passive reference dyes 16 1.8 Contamination prevention 17

Real Time PCR - Basic simple animation - part 1 intro HD.mp4 Specialized thermal cyclers equipped with fluorescence detection modules are used. The measured fluorescence is proportional to the total amount of amplicon; the change in fluorescence over time is used to calculate the amount of amplicon. How Does Real-Time PCR Work? Real-time PCR (SYBR green) protocol (Briana 6/2011) Principle: PCR technology is widely used for quantifying DNA because the amplification of the target sequence allows for greater sensitivity of detection than other methods. In an optimized reaction, the target quantity will approximately double during each amplification cycle.

5/30/2018 · The advent of real-time PCR and real-time reverse transcription PCR (real-time RT-PCR) has dramatically changed the field of measuring gene expression. Real-time PCR is the technique of collecting data throughout the PCR process as it occurs, thus … Real-time PCR (SYBR green) protocol (Briana 6/2011) Principle: PCR technology is widely used for quantifying DNA because the amplification of the target sequence allows for greater sensitivity of detection than other methods. In an optimized reaction, the target quantity will approximately double during each amplification cycle.

real time pcr principle pdf

Real-Time PCR Basics Thermo Fisher Scientific US